Contact Shop
N4704_AF568_FluoTagX4_Anti_Human_IgG

PFA-fixed Hela cells stably expressing a TOM70-nfRuby-ALFA fusion protein (nf: non-fluorescent) were stained with FluoTag®-X2 anti-mRuby Atto 488 (N3302-At488; dilution: 1:500) and co-stained with a recombinant antibody (rHcAb) anti-ALFA Human IgG1 Fc-fusion (N1586, dilution 1:1000). The anti-ALFA Human IgG1 Fc-fusion rHcAb was detected using FluoTag®-X4 anti-Human IgG conjugated to AZDye568 (N4704-AF568; dilution: 1:500). A Greyscale image of the staining performed with N1586 and N4704-AF568. B Greyscale image of the staining performed with N3302-At488. C The corresponding DAPI signal of the depicted section. D Merge of A, B and C. False color representation of A in red, B in green and C in blue.

 

 N4704_FluoTagX4_Biclonal 2E5+2F3_NanoTag

ELISA plates coated with 0.1 pmol of Human IgG1, IgG2, IgG3 or IgG4 were incubated with a dilution series of the biotinylated sdAb anti-Human IgG two-clone mix (Clones 2E5 + 2F3, mix 1:1). Bound biotinylated sdAbs were decorated with Streptavidin-HRP and detected using TMB reagent. This assay demonstrates the specificity of the two-clone blend, which is available as the fluorescently labeled FluoTag®-X4 anti-Human IgG (Cat. No. N4704). Both clones are also available separately in non-conjugated or non-fluorescent forms (Clone 2E5: Cat. No. N4706, Clone 2F3: Cat. No. N4705).

 N4706-Biotin (Clone 2E5)_NanoTag

ELISA plates coated with 0.1 pmol of Human IgG1, IgG2, IgG3 or IgG4 were incubated with a dilution series of biotinylated sdAb anti-Human IgG, clone 2E5 (Cat. No. N4706-Biotin). Bound biotinylated sdAb was decorated with Streptavidin-HRP and detected using TMB reagent. Note that clone 2E5 exhibits the highest affinity for Human IgG1 and IgG4, a lower affinity for Human IgG2 and only weak binding to IgG3.

 N4705-Biotin (Clone 2F3)_NanoTag

ELISA plates coated with 0.1 pmol of Human IgG1, IgG2, IgG3 or IgG4 were incubated with a dilution series of biotinylated sdAb anti-Human IgG, clone 2F3 (Cat. No. N4705-Biotin). Bound biotinylated sdAb was decorated with Streptavidin-HRP and detected using TMB reagent. Note that clone 2F3 binds to Human IgG1, IgG3 and IgG4 with similar affinity, but does not bind to IgG2.
N4704_AF568_FluoTagX4_Anti_Human_IgG
N4704_FluoTagX4_Biclonal 2E5+2F3_NanoTag
N4706-Biotin (Clone 2E5)_NanoTag
N4705-Biotin (Clone 2F3)_NanoTag

FluoTag®-X4 anti-Human IgG

Cat No: N4704 Category:

195,00 €

FluoTag®-X4 anti-Human IgG is an effective sdAb-based secondary reagent  from our Smart Secondaries®  product line. It is conjugated to fluorophores (FluoTag®) and specifically targets Human IgG immunoglobulins, making it particularly well-suited for  One-step immunofluorescence.

Read more

FluoTag®-X4 anti-Human IgG is a is a blend of two distinct single-domain antibody (sdAb, nanobody, VHH) clones, each carying two fluorophores. These sdAbs can simultaneously bind to the Fc region of Human IgG. The reagent recognizes all four IgG isotypes but exhibits exceptionally high affinity for Human IgG1 and IgG4 (see Figures). This makes it an ideal secondary tool for high-precision imaging applications involving primary human antibodies of the IgG1 or IgG4 isotype. As part of our Smart Secondaries® range, the FluoTag®-X4 anti-Human IgG is particularly suited for One-step immunofluorescence (One-step IF).

To learn more about FluoTags®, please visit our Technology section here.

We provide this product conjugated to various fluorophores and offer custom conjugation services with most commercially available fluorophores, provided these are available in a thiol-reactive activated form.

MSDS
Protocols
Reconstitution and Storage
Variations:
Conjugation Amount Cat No. RRID
Atto488 500 μl N4704-At488-S AB_3676472
AZDye568 500 μl N4704-AF568-S AB_3676473
AbberiorStar635P 500 μl N4704-Ab635P-S AB_3676476
Atto643 500 μl N4704-At643-S AB_3676474
Alexa647 500 μl N4704-AF647-S AB_3676475
LI-COR IRDye 680RD 500 μl N4704-Li680-S AB_3676477
LI-COR IRDye 800CW 500 μl N4704-Li800-S AB_3676478
Related Products:

sdAb anti-Human IgG, clone 2E5 (Cat. No. N4706)

sdAb anti-Human IgG, clone 2F3 (Cat. No. N4705)
Clone: 2F3, 2E5
Host: Alpaca
Produced in: E.coli
Application: FACS, IF, IHC, WB
Dilution: 1:500 (corresponding to 5 nM final concentration for each clone if reconstituted as proposed below).
Capacity: N/A
Antigen: -
Targets: Human IgG
Specificity: Recognizes the Fc domain of native Human IgGs. Human IgG1 +++, Human IgG2 +, Human IgG3 ++, Human IgG4 +++. No significant cross-reaction with Mouse, Rabbit, GuineaPig, Chicken, Rat, Goat IgGs or Human IgA, IgD, IgE or IgM.
Formulation:

A mixture of two sdAb clones lyophilized from PBS pH 7.4, containing 2% BSA (US-Origin). Reconstitute with 500 µL of 50% glycerol in deionized water. We recommend including 0.1% sodium azide as a preservative if applicable. When reconstituted with 500 µl, the concentration of each single-domain antibody clone is 2.5 µM. For more details, click the Protocols button above and check Reconstitution and Storage.
kDa: -
Ext Coef: -
Shipping: Ambient temperature
Storage:

Vials containing lyophilized reagent can be stored at 2-8°C for up to 12 months. After reconstitution, store at -80°C for up to 6 months. Working aliquots can be stored at -20°C for up to 4 weeks. For more details, click the Protocols button above and check Reconstitution and Storage.
Protocols:

Relevant protocols can be found under the Protocols button above. For additional information, visit our Resources page.
References: -
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
Legal terms: By purchasing this product you agree to our general terms and conditions.

Related products