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N4702_At488_FluoTag®-X2 anti-Human IgG_NanoTag

Methanol-fixed HeLa cells stained with anti-Actin (human recombinant monoclonal IgG1; Abcam ab213251; dilution 1:300) and detected with FluoTag®-X2 anti-Human IgG conjugated to Atto 488 (Cat. No. N4702-At488; dilution 1:500). A Staining with anti-Actin and N4702-At488. B DAPI signal. C Merge of A and B. D Control without anti-Actin antibody.

 

 N4705-Biotin (Clone 2F3)_NanoTag

ELISA plates coated with 0.1 pmol of human IgG1, IgG2, IgG3 or IgG4 were incubated with a dilution series of biotinylated sdAb anti-Human IgG, clone 2F3 (Cat. No. N4705-Biotin). Bound biotinylated sdAb was decorated with Streptavidin-HRP and detected using TMB reagent. Note that clone 2F3 binds to human IgG1, IgG3 and IgG4 with similar affinity, but does not bind to IgG2.

 N4702_AF568_FluoTag®-X2 anti-Human IgG_NanoTag

Methanol-fixed HeLa cells stained with anti-Actin (human recombinant monoclonal IgG1; Abcam ab213251; dilution 1:300) and detected with FluoTag®-X2 anti-Human IgG conjugated to AZDye 568 (Cat. No. N4702-AF568; dilution 1:500). A Staining with anti-Actin and N4702-AF568. B DAPI signal. C Merge of A and B. D Control without anti-Actin antibody.

 N4702_Ab635P_FluoTag®-X2 anti-Human IgG_NanoTag

Methanol-fixed HeLa cells stained with anti-Actin (human recombinant monoclonal IgG1; Abcam ab213251; dilution 1:300) and detected with FluoTag®-X2 anti-Human IgG conjugated to abberior STAR 635P (Cat. No. N4702-Ab635P; dilution 1:500). A Staining with anti-Actin and N4702-Ab635P. B DAPI signal. C Merge of A and B. D Control without anti-Actin antibody.

 N4702_At643_FluoTag®-X2 anti-Human IgG_NanoTag

Methanol-fixed HeLa cells stained with anti-Actin (human recombinant monoclonal IgG1; Abcam ab213251; dilution 1:300) and detected with FluoTag®-X2 anti-Human IgG conjugated to Atto 643 (Cat. No. N4702-At643; dilution 1:500). A Staining with anti-Actin and N4702-At643. B DAPI signal. C Merge of A and B. D Control without anti-Actin antibody.

 N4702_AF647_FluoTag®-X2 anti-Human IgG_NanoTag

Methanol-fixed HeLa cells stained with anti-Actin (human recombinant monoclonal IgG1; Abcam ab213251; dilution 1:300) and detected with FluoTag®-X2 anti-Human IgG conjugated to Alexa Fluor 647 (Cat. No. N4702-AF647; dilution 1:500). A Staining with anti-Actin and N4702-AF647. B DAPI signal. C Merge of A and B. D Control without anti-Actin antibody.
N4702_At488_FluoTag®-X2 anti-Human IgG_NanoTag
N4705-Biotin (Clone 2F3)_NanoTag
N4702_AF568_FluoTag®-X2 anti-Human IgG_NanoTag
N4702_Ab635P_FluoTag®-X2 anti-Human IgG_NanoTag
N4702_At643_FluoTag®-X2 anti-Human IgG_NanoTag
N4702_AF647_FluoTag®-X2 anti-Human IgG_NanoTag

FluoTag®-X2 anti-Human IgG

Cat No: N4702 Category:

195,00 €

FluoTag®-X2 anti-Human IgG is an effective sdAb-based secondary reagent  from our Smart Secondaries® product line. It is conjugated to fluorophores (FluoTag®) and specifically targets human IgG immunoglobulins, making it particularly well-suited for OneStep Immunofluorescence.

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FluoTag®-X2 anti-Human IgG is a single-domain antibody (sdAb, nanobody, VHH) clone (2F3), carying two fluorophores. This sdAb binds to the Fc region of human IgG. The reagent recognizes human IgG1, IgG3 and IgG4 isotypes with exceptionally high affinity. This makes it an ideal secondary tool for high-precision imaging applications involving primary human antibodies of the IgG1, IgG3 or IgG4 isotype. As part of our Smart Secondaries® range, the FluoTag®-X2 anti-Human IgG is particularly suited for OneStep Immunofluorescence (OneStep IF).

To learn more about FluoTags®, please visit our Technology section here.

We provide this product conjugated to various fluorophores and offer custom conjugation services with most commercially available fluorophores, provided these are available in a thiol-reactive activated form.

MSDS
Protocols
Reconstitution and Storage
Variations:
Conjugation Amount Cat No. RRID
Atto488 500 μL N4702-At488-S AB_3676472
AZDye568 500 μL N4702-AF568-S AB_3676473
abberior STAR 635P 500 μL N4702-Ab635P-S AB_3676476
Atto643 500 μL N4702-At643-S AB_3676474
Alexa647 500 μL N4702-AF647-S AB_3676475
LI-COR IRDye 680RD 500 μL N4702-Li680-S AB_3676477
LI-COR IRDye 800CW 500 μL N4702-Li800-S AB_3676478
Related Products:

sdAb anti-Human IgG, clone 2F3 (Cat. No. N4705)

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Clone: 2F3
Host: Alpaca
Produced in: E.coli
Application: FACS, IF, IHC, WB
Dilution: 1:500 (corresponding to 5 nM final concentration if the sdAb was reconstituted as proposed below)
Capacity: N/A
Antigen: -
Targets: Human IgG
Specificity: Recognizes the Fc domain of native Human IgGs. Human IgG1 +++, Human IgG3 +++, Human IgG4 +++. No significant cross-reaction with Mouse, Rabbit, GuineaPig, Chicken, Rat, Goat IgGs or Human IgA, IgD, IgE or IgM.
Formulation:

The single sdAb clone was lyophilized from PBS pH 7.4, containing 2% BSA (US-Origin). Reconstitute with 500 µL of 50% glycerol in deionized water. We recommend including 0.1% sodium azide as a preservative if applicable. When reconstituted with 500 µL, the single-domain antibody concentration is 5 µM. For more details, click the Protocols button above and check Reconstitution and Storage.
kDa: -
Ext Coef: -
Shipping: Ambient temperature
Storage:

Vials containing lyophilized reagent can be stored at 2-8°C for up to 12 months. After reconstitution, store at -80°C for up to 6 months. Working aliquots can be stored at -20°C for up to 4 weeks. For more details, click the Protocols button above and check Reconstitution and Storage.
Protocols:

Relevant protocols can be found under the Protocols button above. For additional information, visit our Resources page.
References: -
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
Legal terms:

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