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anti-ALFA x anti-IgG Bispecifics_NanoTag Biotechnologies

The anti-ALFA × anti-IgG Bispecifics are fusions of the sdAb anti-ALFA (NbALFA) and a secondary single-domain antibody (2°sdAb) recognizing primary antibodies. Their dual specificity enables the simultaneous binding of ALFA-tagged effectors (e.g., enzymes) and primary IgG antibodies from rabbits or mice, allowing the assembly of modular ternary effector complexes. Different variants are available based on the species and isotype specificity of the 2°sdAb moiety.

anti-ALFA x anti-IgG Bispecifics

Cat No: N1590 Category:

275,00 €

anti-ALFA x anti-IgG Bispecifics enable simultaneous binding of ALFA-tagged targets and various mouse or rabbit IgG subtypes. This modular system opens up opportunities to explore multivalent interactions, facilitating diverse experimental applications with enhanced versatility and precision.

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Bispecific single-domain antibodies represent a groundbreaking advancement in the field of immunotherapy and targeted drug delivery. These innovative molecules are engineered to bind simultaneously to two distinct antigens or epitopes, which can be particularly advantageous for treating complex diseases like cancer and autoimmune disorders.

Our anti-ALFA x anti-IgG Bispecifics are fusions of the sdAb anti-ALFA (NbALFA) and a secondary single-domain antibody (2°sdAb) recognizing primary antibodies (see Figure). Due to their dual specificity, the anti-ALFA x anti-IgG Bispecifics enable simultaneous binding of ALFA-tagged effectors (e.g., enzymes) and primary IgG antibodies from rabbit or mouse. They therefore provide versatile linkers moieties for assembly of modular ternary effector complexes involving primary antibodies and ALFA-tagged effector units. We offer various anti-ALFA x anti-IgG Bispecifics depending on the species and isotype specificity of the 2°sdAb moiety.

MSDS
Protocols
Reconstitution and Storage
Variations:
Conjugation Amount Cat No. RRID
anti-Mouse IgG1 250 μg N1590-250ug AB_3668673
anti-Mouse IgG2a/b 250 μg N1591-250ug AB_3668674
anti-Rabbit IgG 250 μg N1592-250ug AB_3668675
Related Products: -
Clone: ALFA 1G5; msIgG1 10A4; msIgG2 14A4; rbIgG 10E10
Host: Alpaca
Produced in: E.coli
Application: ELISA, FACS
Dilution: N/A
Capacity: N/A
Antigen: -
Targets: ALFA-tag, ALFA/msIgG1, ALFA/msIgG2a/b, ALFA/rbIgG
Specificity: The anti-ALFA sdAb recognizes the ALFA-tag (SRLEEELRRRLTE). Each anti-Mouse IgG, or anti-Rabbit IgG sdAb recognizes either native mouse IgG1, mouse IgG2a and IgG2b, or rabbit IgG, respectively. The anti-Mouse IgG1 and anti-Mouse IgG2a/b sdAbs do not cross-react with Guinea Pig, Rat, Rabbit, Chicken, Donkey, Goat, Pig, Horse, Cow or Human IgGs. The anti-Rabbit IgG sdAb does not cross-react with Mouse, Guinea Pig, Chicken, Rat, Donkey, Goat, Pig, Horse, Cow or Human IgGs.
Formulation:

Anti-ALFA x anti-IgG Bispecific sdAbs are lyophilized from PBS pH 7.4. Reconstitute with 250 µL sterile 50% glycerol (v/v) in deionized water for storage at -80°C. For more details, click the Protocols button above and check Reconstitution and Storage.
kDa: -
Ext Coef: -
Shipping: Ambient temperature
Storage:

Vials containing lyophilized reagent can be stored at 2-8°C for up to 12 months. After reconstitution, store at -80°C for up to 6 months. Working aliquots can be stored at -20°C for up to 4 weeks. For more details, click the Protocols button above and check Reconstitution and Storage.
Protocols:

Relevant protocols can be found under the Protocols button above. For additional information, visit our Resources page.
References: -
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
Legal terms: By purchasing this product you agree to our general terms and conditions.

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