Contact Shop
ALFA Peptide Resin 10ml_N1530_N1535_NanoTag
ALFA Peptide Resin_N1530_NanoTag Biotechnologies

Affinity purification of NbALFA (sdAb anti-ALFA) using ALFA Peptide Resin (Cat. No. N1530). 2.5 µM NbALFA was spiked into E. coli lysate and applied twice in flow over 0.5 mL ALFA Peptide Resin at room temperature. After binding, the resin was washed with PBS and eluted in flow with five column volumes of sodium citrate buffer at pH 2.2 (A), pH 3.0 (B), or pH 5.0 (C) in separate experiments. Remaining bound protein was eluted with SDS sample buffer. Indicated fractions were analyzed by SDS–PAGE followed by Coomassie Blue staining. The pH 5.0 elution served as a negative control.
ALFA Peptide Resin 10ml_N1530_N1535_NanoTag
ALFA Peptide Resin_N1530_NanoTag Biotechnologies

ALFA Peptide Resin

Cat No: N1530 Category:

Price range: 420,00 € through 685,00 €

ALFA Peptide Resin is a high-affinity resin for efficient and clean purification of proteins fused to NbALFA (sdAb anti-ALFA). It features an ALFA-like peptide covalently immobilized on 4% agarose or magnetic agarose beads.

Read more

The ALFA® system is based on the ALFA-tag, a highly versatile epitope tag with the core sequence SRLEEELRRRLTE, and its high-affinity binding partner, NbALFA (sdAb anti-ALFA), a single-domain antibody (sdAb) with a dissociation constant of approximately 26 pM .

ALFA Peptide Resins are affinity resins specifically designed for the selective purification of proteins fused to NbALFA. The resins are available in two formats, based on 4% cross-linked agarose or magnetic agarose. Both variants feature an immobilized ALFA-like peptide that enables the efficient and highly specific capture of NbALFA and NbALFA-tagged protein of interest (POI). Elution is performed under mild acidic conditions (pH 3.0), which typically preserves the native conformation and functionality of sensitive proteins by avoiding harsh denaturing or reducing agents. This makes ALFA Peptide Resins particularly suitable for applications requiring functional, structurally intact proteins.

ALFA Peptide Resin_NanoTag Biotechnologies

Both ALFA Peptide Resin variants are compatible with various buffer compositions during binding and washing, and tolerate high concentrations of common detergents and reducing agents (see Protocols for details), offering flexibility for customized purification workflows. Due to the covalent attachment of the ALFA-like peptide, the resins can withstand multiple regeneration cycles and harsh cleaning-in-place (CIP) procedures without significant loss of performance.

See our Technology section for more information about the ALFA® system.

MSDS
Protocols
Manual ALFA Peptide Resin
Variations:
Conjugation Amount Cat No. RRID
4% cross-linked agarose 10 mL slurry N1530 -
4% cross-linked magnetic agarose 10 mL slurry N1535 -
Related Products:

 

 

View related products
Clone: -
Host: -
Produced in: Synthetic peptide
Application: IP
Dilution: N/A
Capacity: Binding capacity depends on target hydrodynamic properties, e.g., for NbALFA (14 kDa) binding capacity ~500 µM (~7 mg/mL); for NbALFA–Halo fusion (48 kDa) binding capacity ~400 µM (~19 mg/mL).
Antigen: -
Targets: NbALFA
Specificity: Specific binding to NbALFA (sdAb anti-ALFA)
Formulation:

50% slurry in PBS containing 20% Ethanol
kDa: -
Ext Coef: -
Shipping: Ambient temperature
Storage:

Store at 4°C for up to 12 months. Do not freeze!
Protocols:

Protocol for IP and elutions using the ALFA Peptide Resin can be found under the Protocols button above. For additional information, visit our Resources page.
References:
  1. Götzke H, Kilisch M, Martínez-Carranza M, Sograte-Idrissi S, Rajavel A, Schlichthaerle T, Engels N, Jungmann R, Stenmark P, Opazo F, Frey S. The ALFA-tag is a highly versatile tool for nanobody-based bioscience applications. Nat Commun. 2019 Sep 27;10(1):4403. doi: 10.1038/s41467-019-12301-7. PMID: 31562305; PMCID: PMC6764986.
  2. Kilisch M, Götzke H, Gere-Becker M, Crauel A, Opazo F, Frey S. Discovery and Characterization of an ALFA-Tag-Specific Affinity Resin Optimized for Protein Purification at Low Temperatures in Physiological Buffer. Biomolecules. 2021;11(2):269. Published 2021 Feb 12. doi:10.3390/biom11020269 (IP; HeLa)
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
Legal terms:

By purchasing this product you agree to our general terms and conditions.