A novel FluoTag® variant for simplified and precise SMLM
Say goodbye to buffer optimization – get spontaneous blinking right in PBS!
Why Choose FluoTag® Janelia Fluor® 635b?
- Spontaneous blinking in PBS: JF635b cycles automatically between OFF and ON states – no need for photoactivation or redox buffers!
- Ideal duty cycles for resolving both lower and abundant targets.
- Superior photostability and brightness: Suitable for longer imaging sessions, super-resolution microscopy including single-molecule localization microscopy (SMLM).
- Spectral properties: Excitation λ 651 nm, Emission λ 667 nm. The red-shifted emission for reduced background autofluorescence.
Read the detailed characterization of the dye by Holland et al. 2024 BioRxiv “A series of spontaneously blinking dyes for super-resolution Microscopy”.
Elevate Your SMLM by Combining the Benefits of sdAbs and JF635b
FluoTags® conjugated to JF635b available now:
- FluoTag®-X2 anti-ALFA (Cat. No. N1502-JF635B-L)
- FluoTag®-X4 anti-GFP (Cat. No. N0304-JF635B-L)
- FluoTag®-X2 anti-mEos (Cat. No. N3102-JF635B-L)
More coming soon (including our Smart Secondaries®)…
What Super-Resolution Microscopy Experts Are Saying After Using FluoTag® JF635b
“…the FluoTag®-X2 anti-mEos JF635b works like a charm!” — C.G. Specht, INSERM, France.
“ I tried the FluoTag®-X2 anti-ALFA JF635b on the FtsZ-ALFA E. coli strain (on the chromosome as only copy) using STORM, and it worked soooooo well! No special buffer, just fixed as usual, then mounted.” — B. Söderström, AIMI, Australia.